Synthetic methylated CpG ODNs are potent in vivo adjuvants when delivered in liposomal nanoparticles

Synthetic methylated CpG ODNs are potent in vivo adjuvants when delivered in liposomal nanoparticles.

Although it is well documented that the immunological activity of cytosine-guanine (CpG) motifs is abrogated by 5' methylation of the cytosine residue, encapsulation within stabilized lipid nanoparticles endows these methylated cytosine-guanine- (mCpG-) containing oligonucleotides (ODNs) with potent immunostimulatory activity in murine animal models. Surprisingly, not only do liposomal nanopart...

Encephalitogenic T Cells In Vivo for the Activation of Autoreactive CpG Oligonucleotides Are Potent Adjuvants

Comparison of in vivo Adjuvanticity of Liposomal PO CpG ODN with Liposomal PS CpG ODN: Soluble Leishmania Antigens as a Model

Objective(s)CpG oligodeoxynucleotides (CpG ODNs) have been shown to have potent immunostimulatory adjuvant activity for a wide range of antigens. Due to susceptibility of phosphodiester CpG ODNs (PO CpG) to nuclease degradation, nuclease-resistant phosphorothioate CpG ODNs (PS CpG) were currently utilized in an in vivo model. In this study, according to some recently reported drawbacks with PS ...

Liposome-Encapsulated Adjuvants are Potent Inducers of Antigen- Specific T-Cells in Vivo

As shown previously, encapsulation of a peptide derived from tyrosinase-related protein2 (TRP2) into liposomes (artificial virus envelope (AVE) 3) resulted in combination with CpG-oligodeoxynucleotides in the induction of higher numbers of antigen-specific T cells compared to vaccination with free TRP2. Here, we present further data with regard to optimal antigen dose, the relevance of vaccine ...

comparison of in vivo adjuvanticity of liposomal po cpg odn with liposomal ps cpg odn: soluble leishmania antigens as a model

objective(s)cpg oligodeoxynucleotides (cpg odns) have been shown to have potent immunostimulatory adjuvant activity for a wide range of antigens. due to susceptibility of phosphodiester cpg odns (po cpg) to nuclease degradation, nuclease-resistant phosphorothioate cpg odns (ps cpg) were currently utilized in an in vivo model. in this study, according to some recently reported drawbacks with ps ...